ABSTRACT
Nanoemulsion-encapsulated lzp3 DNA vaccine (pCDNA3-Aat-COMP-lzp3-C3d3, lzp3) was prepared, and its quality was evaluated. The interfacial emulsification method was employed to make the nanoemulsion-encapsulated plasmid, and its sizes and distribution were detected by electron microscope. Anion exchange chromatography was used to separate the nanoemulsion from the plasmid based on the charge characteristics of plasmid. The determination method for encapsulation efficiency of the plasmid nanoemulsion was established by anion exchange chromatography. The results indicated that the average size of the plasmid nanoemulsion is (23 +/- 10) nm, and the encapsulation efficiency is 80.5%. The separation and determination conditions of nanoemulsion-encapsulated plasmid was as follows: eluent buffer was 0.05 mol/L Tris-HCl solution at a flow rate of 0.7 mL/min, while detection wavelength for plasmid was 260 nm, the temperature of column was maintained at 30 degrees C, and injection volume was 2mL. On this condition, nanoemulsion and plasmid can be separated on the column of anion exchange chromatography significantly. This method is simple, sensitive and reproducible with respect to good linearity in the range of 0.05-0.80 mg/mL (r = 0.9983), which can be applied to determine the entrapment efficiency of nanoemulsion-encapsulated plasmid.
Subject(s)
Animals , Rats , Chromatography, Ion Exchange , Methods , Drug Carriers , Chemistry , Emulsions , Nanotechnology , Plasmids , Chemistry , Vaccines, DNA , Chemistry , Zona Pellucida , Allergy and ImmunologyABSTRACT
<p><b>AIM</b>To investigate the preparation, shape and ultrasound triggered release characteristics of gelatin nanogel modified with fluoride anion.</p><p><b>METHODS</b>Adriamycin gelatin nanogel modified with fluoride anion (ADM-FMNG) was prepared by co-precipitation with fluoride anion. The content and encapsulation rate of adriamycin were measured by HPLC method. The size and shape of ADM-FMNG were determined by electron microscope. The size and distribution of ADM-FDNG before and after sonication were measured by laser size analysis device.</p><p><b>RESULTS</b>The average diameter of ADM-FMNG was (46 +/- 12) nm. Adriamycin encapsulated rate and loading were 87.2% and 0.091 g x L(-1), respectively. 48.5% of adriamycin was released within 50 h while in vitro at 37 degrees C. Under the action of ultrasound that has the frequency of 20 kHz, 0.4 W x cm(-2) of power density and 7-8 min duration, 51. 5% of adriamycin in ADM-FMNG was released that was significantly higher than control group, the size of ADM-FMNG was changed from (46 +/- 12) nm to (1,212 +/- 35) nm and restored after ultrasound stopped for 3-4 min.</p><p><b>CONCLUSION</b>ADM-FDNG system has the sensitive ultrasound triggered release characteristics.</p>
Subject(s)
Doxorubicin , Drug Carriers , Drug Delivery Systems , Fluorides , Gelatin , Nanoparticles , Particle Size , Polyethylene Glycols , Chemistry , Polyethyleneimine , Chemistry , UltrasonicsABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ultrasonic wave on extracting Konjac Glucomannan(KGM) in Konjac refined powder.</p><p><b>METHOD</b>Free reduced sugar in Konjac refined powder was removed and Konjac refined powder in the aqueous solution was processed by ultrasonic wave and KGM content was measured by spectrophotometry.</p><p><b>RESULT</b>KGM content in the Konjac refined powder aqueous solution by ultrasonic process at fixed 40 kHz, 100 W, 30-45 min was equal to that by routine method at 4 h; whereas, by 1 h of ultrasonic process, KGM content was significantly enhanced than that by 4 h of routine method(P < 0.01), enhancement rate was 6.5%. Linearity of standard glucose was good (r = 0.9996) in range of 0.2-1.6 mg. The average recovery was 97.8%, RSD of repeatability was 1.27%.</p><p><b>CONCLUSION</b>Ultrasonic extraction in aqueous solution is a reliable and rapid method that can enhance extraction efficiency of KGM in Konjac refined powder.</p>
Subject(s)
Amorphophallus , Chemistry , Mannans , Powders , UltrasonicsABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative method for the content determination and monosaccharide composition analysis of Konjac glucomannan (KGM) in Konjac refined powder by pre-column derivatization high performance liquid chromatographic method (HPLC).</p><p><b>METHOD</b>The two derivatives combined reducing monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) were separated by reverse-phase HPLC using a developed fragment gradient elution process, and monitored by ultraviolet detector at 250 nm. The broad reagent peak of PMP was separated very well from all the PMP-sugars, and good separation was achieved for derivatives of mannose and glucose. The quantitative methods of two reducing monosaccharides were studied by the method combined internal and external standard; while the KGM content in Konjac refined powder was determined.</p><p><b>RESULT</b>Linearity of glucose was good (r = 0.9990) in range of 1.002-8.016 nmol; while mannose (r = 0.9994) in range of 1.001-8.008 nmol. The average recovery of this method was 98.1%, RSD of repeatability was 1.72%. KGM content in Konjac refined powder was 79.5%, ratio of glucose to mannose in KGM was 1:1.51.</p><p><b>CONCLUSION</b>This method is a sample, convenient and rapid method that can determine KGM content and analyze monosaccharide compositions in KGM, which will be helpful to quality assessment of Konjac refined powder.</p>